Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Pre-treatment with 180 uM cycloheximide for 30s. PBS wash. For fractionated cells, release cytosol with digitonin. Extract remaining sample with dodecyl maltoside. NEB Directional Small RNA kit PNK end polishing, linker ligation, reverse transcription, PCR amplification